Flow Cytometry and Cell Sorting Facility

Overview

Flow cytometry is the science of examining physical and chemical properties of live cells, beads or other biological particles as they pass in a fluid stream through a measuring apparatus. This apparatus, known as a flow cytometer, uses laser excitation and signal detection to measure parameters such as size, shape, DNA content, surface receptors, enzyme activity, membrane permeability and calcium flux. Some flow cytometers, called sorters, are equipped to separate and collect cells or beads of interest. High-performance flow cytometers and high-speed cell sorters have applications in cancer and HIV research, drug discovery, stem cell and gene therapy.

The Ottawa Hospital Research Institute Flow Cytometry Facility, located at the General Campus of the Ottawa Hospital, was established in 1998 and now includes three flow cytometers, two of which have sorting capabilities. We offer the sorting precision required for the isolation of rare cell populations with high purity and yield, up to 20 000 events per second. As well as sorting, flow cytometers allow the simultaneous analysis of a variety of light scatter and fluorescence parameters on single cells.

Please follow the Sample Submission Guidelines when using the Flow Cytometry Facility services.

Important Announcement

On March 1, 2018, StemCore implemented an online core facility management system to streamline the process of ordering and billing for core service requests. Initially, the system will only include samples submitted to the DNA Sequencing and Flow Cytometry Facilities. On April 1, 2018 we will expand the system to include all services that are offered by StemCore.

Access to the iLab site requires a one-time registration. Once you are registered, the system will enable you to place service requests, provide required approvals for work to be performed, and monitor progress of the requested work.

To complete the registration process, please follow the instruction under the Getting Started with iLab section below. In the event that you already have an iLab account please navigate to the StemCore page within iLab to create equipment reservations or to request services.

If you encounter any difficulties while navigating the iLab site please contact the Facility Coordinator, Fernando Ortiz.