Flow Cytometry and Cell Sorting

Services Provided

Flow cytometry

  • Immunophenotyping/immunofluorescence by monoclonal antibodies.
  • Total/Absolute population counts.
  • Analysis of surface and intracellular fluorescent labelling, simultaneously.
  • Monitoring gene transfer and expression using fluorescent proteins.
  • Simultaneous detection and sorting of GFP and YFP.
  • DNA content and cell cycle analysis using PI, Hoechst, BrDU, etc.
  • Hoechst 33342 side population.
  • Cell viability with exposure to drugs, toxins, etc.
  • Apoptosis.
  • Caspase activity.
  • Telomere length measurement.
  • Neutrophil functions (phagocytosis and oxidative burst).
  • NK cytolytic activity.
  • MLR / CTL cytotoxicity.
  • Caspase activity.
  • Mitochondrial function (DiO, JC-1)
  • Intracellular Calcium Flux.
  • Q-Prep whole blood lysis.

Cell Sorting

  • High-speed cell sorting.
  • Cell sorting of one to four subpopulations.
  • Large particle sorting
  • Single cell deposition in multi-well plates.

Antibody Testing and Compensation matrix

  • Additional service is offered for antibody testing of mouse, rat and hamster origin using antibody capture beads. This beads are a useful tool to detect the intensity of your antibodies and to calculate the compensation matrix in experiments with more than 6 parameters.

Training

  • Flow cytometry basic training is intended for students, technicians and investigators wanting to strengthen their background and understanding of flow cytometry. The 2-hour session will provide the participants with the required tools for setting up successful flow cytometry experiments.
  • Specific Flow Cytometry training is offered on an as-needed basis; please contact the facility coordinator for further details.