Scientific Publications Database
Article Title: Homocysteine levels associate with subtle changes in leukocyte DNA methylation: an epigenome-wide analysisAuthors: Mandaviya, Pooja R.; Aissi, Dylan; Dekkers, Koen F.; Joehanes, Roby; Kasela, Silva; Vinh Truong; Stolk, Lisette; van Heemst, Diana; Ikram, M. Arfan; Lindemans, Jan; Slagboom, P. Eline; Tregouet, David-Alexandre; Uitterlinden, Andre G.; Wei, Chen; Wells, Phil; Gagnon, France; van Greevenbroek, Marleen M. J.; Heijmans, Bastiaan T.; Milani, Lili; Morange, Pierre-Emmanuel; van Meurs, Joyce B. J.; Heil, Sandra G.
Journal: EPIGENOMICS Volume 9 Issue 11
Date of Publication:2017
Abstract:
Aim: Homocysteine (Hcy) is a sensitive marker of one-carbon metabolism. Higher Hcy levels have been associated with global DNA hypomethylation. We investigated the association between plasma Hcy and epigenome-wide DNA methylation in leukocytes. Methods: Methylation was measured using Illumina 450 k arrays in 2035 individuals from six cohorts. Hcy-associated differentially methylated positions and regions were identified using meta-analysis. Results: Three differentially methylated positions cg21607669 (SLC27A1), cg26382848 (AJUBA) and cg10701000 (KCNMA1) at chromosome 19, 14 and 10, respectively, were significantly associated with Hcy. In addition, we identified 68 Hcy-associated differentially methylated regions, the most significant of which was a 1.8-kb spanning domain (TNXB/ATF6B) at chromosome 6. Conclusion: We identified novel epigenetic loci associated with Hcy levels, of which specific role needs to be further validated.