Laboratory Services (prices are effective as of April 1, 2017)

Service Fee if facility does work for uOttawa / affiliate lab Fee if user from uOttawa / affiliate lab does work Fee if facility does work for external client (other academic institutions, government or private)
Training $50/hr $50/hr $60/hr
Technician time $25/hr None applicable $50/hr
Reprogramming $500/sample None applicable $1000/sample
Harvesting/freezing $20/sample None applicable $40/sample
Differentiation Value is variable due to length of experiment None applicable Value is variable due to length of experiment
Characterization and analysis (Cellomics) $150/12 well plate None applicable $300/12 well plate
Housing Value is variable due to length of experiment Value is variable due to length of experiment Value is variable due to length of experiment
Teratoma injection $40 per injection $40 per injection $80 per injection
Teratoma harvesting $40 per teratoma $40 per teratoma $80 per teratoma
Consumables $400/month $400/month $600/month

Differentiation

We have successfully differentiated the following cell types (see below) from iPSCs and/or ESCs. Training on how to differentiate iPSCs and ESCs is available at the facility. Prices vary depending on the protocol used for differentiation.

  • Smooth Muscle Cells
  • Vascular Smooth Muscle Cells
  • Chondroprogenitors Cells
  • Cardiomyocytes
  • Neuron Progenitor Cells (NPCs)
  • Neurons
  • Neural Crest Cells

CRISPR/Cas9 genome editing

We have developed the protocols necessary to perform edits in pluripotent stem cells using a number of different strategies. For instance, in addition to relying on random insertions/deletions through non-homologous end joining (NHEJ) to knockout a gene of interest, we can pair a Cas9/sgRNA vector with a single stranded DNA template for precision knockout. We have also successfully inserted large transgenes containing selection markers to knockout your gene of interest. We also have all of the tools and vectors needed for gene knock-in at the AAVS1 safe harbor locus for sustained expression. Please contact for more information.