Qubit Fluorometer

StemCore Facility is currently using iLab core management software to streamline the process of ordering and billing for core services. If you do not have an iLab account, please refer to the registration instructions on the StemCore Home Page. If you already have an iLab account please log into StemCore's iLab site to submit your samples.

Overview

The Qubit is a benchtop fluorometer designed to accurately measure DNA and RNA quantity, more sensitive than UV absorbance–based quantification, making it ideal for precious samples

Equipment
Qubit Fluorometer
Qubit 3.0 Fluorometer (Thermo)
Services Provided

StemCore carries the following Qubit Assays:

  • Qubit™ RNA HS Assay Kit (250 pg/µL and 100 ng/µL)
  • Qubit™ dsDNA HS Assay Kit (10 pg/µL to 100 ng/µL)
Process
  • Samples from local researchers should be dropped off at the StemCore lab Facility Monday-Friday between the hours of 9:00 AM and 4:00 PM along with a fully completed Request Form. Please ensure that the label on the tube exactly matches the sample name given on the request form.
  • Samples from external sources should be shipped to the mailing address below via Federal Express or Purolator to the attention of Kathy Sheikheleslamy. In advance of shipment please email the request to ksheikheleslamy@ohri.ca. If your sample has been obtained from human subjects, please include a copy of the appropriate ethics review approval forms. RNA samples should be shipped on dry ice to the StemCore Laboratory. It is important to label the sample "Perishable". Please also include a copy of the previously faxed request form with the shipped samples.
  • Results can generally be obtained 2 days from date of receipt and will be forwarded in a .pdf report by email.
  • All samples are processed on a first come first served basis.
  • Please submit only the required amount as samples are not kept for long term storage and retrieval.
Fees

Qubit quantification fees are $5 per sample. This price is applicable to academic researchers only. Please inquire for large volume or commercial pricing.

Reaction Submission Format

In order to minimize error and reduce turnaround time we will only accept samples that are provided in the following format:

  • Each sample should be provided in a 1.5 ml microfuge tube.
  • The microfuge tube should clearly be labeled with black indelible ink with the sample name as well as the requestor's initials.
  • Please ensure that the name on the microfuge tube corresponds exactly to the name indicated on the request form.
  • 3 ul are required for the Fragment Analyzer assay, 5 ul for both Qubit and Fragment Analyzer.
Mailing Address

Ottawa Hospital Research Institute
Sprott Center for Stem Cell Research
StemCore Laboratories
Room 5132, Critical Care Wing
501 Smyth Road
Ottawa, Ontario, Canada K1H 8L6

Contact Information

Kathy Sheikheleslamy
ksheikheleslamy@ohri.ca
613.737.8899 x73251 (lab phone)
613.739.6294 (fax)