Dilworth Lab

Jeff Dilworth profile picture

Contact Information

Jeff Dilworth, PhD
613-737-8899 ext. 70339

ORCID logo https://orcid.org/0000-0002-6265-8102

Jeff Dilworth

Senior Scientist Affiliate, Regenerative Medicine
Ottawa Hospital Research Institute
Professor, Department of Cellular and Molecular Medicine
University of Ottawa
Professor (Cross-Appointed), Department of Medicine
University of Ottawa
Professor (Cross-Appointed), Department of Surgery
University of Ottawa

Research Interests

Epigenetic regulation of gene expression during mammalian development and regeneration

Brief Biography

Dr Jeff Dilworth received his PhD in 1997 from Queen's University (Kingston, ON) for his research with Dr Glenville Jones examining the metabolism of vitamin D analogs, and how the altered stability of the drugs changed their perceived biological activity in cultured cells. He then carried our his post-doctoral training with Professor Pierre Chambon at the Institut de Génétique et de Biologie Moléculaire et Cellulaire (Strasbourg, France) where he established a chromatin-based in vitro transcription system to characterize the mechanisms through which retinoic acid activates gene expression. As a research associate working with Dr. Stephen Tapscott at the Fred Hutchinson Cancer Research Center (Seattle, WA), he used his background in transcription and epigenetics to begin exploring the mechanisms regulating developmental gene expression programs.

In 2004, Dr Dilworth joined the Regenerative Medicine Program at the Ottawa Hospital Research Institute where he is currently a Senior Scientist within the Sprott Center for Stem Cell Research. His research team works to understand the epigenetic mechanisms controlling cell fate decisions during muscle regeneration, and the role of DNA-bound transcription factors in directing epigenetic enzymes to specific genes for activation of cell-specific gene expression programs. Dr Dilworth also serves as the Chair (Basic Science) of the Research Task Force for the Canadian Neuromuscular Disease Network.

Selected Publications

K. Nakka, S. Hachmer, Z. Mokhtari, R. Kovac, H. Bandukwala, C. Bernard, Y. Li, G. Xie, C. Liu, M. Fahalli, L. Megeney, J. Gondin, B. Chazaud, M. Brand, X. Zha, K. Ge, and F.J. Dilworth. JMJD3 activated hyaluronan synthesis drives muscle regeneration in an inflammatory environment. Science 377: 666-669, 2022.

D. Robinson, M. Ritso, G. Nelson, Z. Mokhtari, K. Nakka, H. Bandukwala, S. Goldman, P. Park, R. Mounier, B. Chazaud, M. Brand, M. Rudnicki, K. Adelman, F.J. Dilworth. Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation. Dev Cell 56: 1014-1029, 2021.

M. Brand, K. Nakka, J. Zhu, and F.J. Dilworth. Polycomb/Trithorax Antagonism: Cellular Memory in Stem Cell Fate and Function. Cell Stem Cell 4: 518-533, 2019.

H. Faralli, C. Wang, A. Benyoucef, S. Sebastian, L. Zhuang, A. Chu, C. Palii, C. Liu, B. Camellato, M. Brand, K. Ge, and F.J. Dilworth. H3K27-demethylase activity of UTX/KDM6A is essential for skeletal muscle regeneration. Journal of Clinical Investigation 126: 1555-1565, 2016.

S. Sebastian, H. Faralli, Z. Yao, P. Rakopoulos, C. Palii, Y. Cao, K. Singh, Q-C. Liu, A. Chu, A. Aziz, M. Brand, S.J. Tapscott, and F.J. Dilworth. Tissue-specific splicing of a ubiquitously expressed transcription factor is essential for muscle differentiation. Genes & Dev 27: 1247-1259, 2013.

S. Rampalli, L. Li, E. Mak, K. Ge, M. Brand, S.J. Tapscott, and F.J. Dilworth. p38 MAPK signaling pathway regulates recruitment of Ash2L-containing methyltransferase complexes to specific genes during differentiation. Nature Struct Mol Biol 14: 1150-1156, 2007.

Diseases, conditions and populations of interest

Research and clinical approaches