Flow Cytometry and Cell Sorting

Instrumentation

BeckmanCoulter MoFlo XDP
Location: Ottawa Hospital Sprott Centre for Stem Cell research, 5th floor Critical Care Wing, room 5145, 501 Smyth Road

The BeckmanCoulter MoFlo XDP is a state-of-the-art instrument, equipped with five excitation laser lines (UV, 405nm, 488nm, 561nm, and 640nm), which can be used for multi-parameter experiments and applications requiring high speed sorting and/or sorting of four sub-populations simultaneously. A designated operator processes the samples.

The MoFlo XDP is a true 32-bit highresolution digital system, which enables accurate sorting rates of up to 70,000 events per second and analysis rates of up to 100,000 events per second. The precision of the electronics platform combined with the reliably stable MoFlo fluidics allows accurate sorting into as much as 1536-well plates.

BeckmanCoulter MoFlo XDP

Configuration

  • Jet-in-air system.
  • Five excitation lasers:
    • Coherent Genesis CX 355nm (UV)
    • Coherent Cube 405nm
    • Coherent Saphire 488nm
    • Coherent Saphire 561nm
    • Coherent Cube 640nm
  • Two light scatter channels and 15 fluorescence detectors:
    • 2 fluorescence detectors on the 355nm laser path
    • 3 fluorescence detectors on the 405nm laser path
    • 5 fluorescence detectors on the 488nm laser path
    • 2 fluorescence detectors on the 561nm laser path
    • 3 fluorescence detectors on the 640nm laser path

A policy has been implement which states that users of the facilily recognize the potential for contamination of their samples and that use of the MoFlo facility includes this assumed risk. The MoFlo does not generally have a high incident rate with contamination issues; however, as with any sample manipulation, the posbility does exist. In the case of post-sorting contamination, it is heavily recommended that the users notify the facility so that proper action can be taken.

Examples of Configurations of the MoFlo XDP

MoFlo XDP configuration using UV laser

MoFlo XDP configuration using Yellow laser