Flow Cytometry and Cell Sorting

Publications

2018

Brun CE, Wang YX, Rudnicki MA. Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells. Methods Mol Biol. 2018;1686:149-159. doi: 10.1007/978-1-4939-7371-2_11.

2017

Hernández-Hernández JM, García-González EG, Brun CE, Rudnicki MA. The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration. Semin Cell Dev Biol. 2017 Dec;72:10-18. doi: 10.1016/j.semcdb.2017.11.010

Fraineau S, Palii CG, McNeill B, Ritso M, Shelley WC, Prasain N, Chu A, Vion E, Rieck K, Nilufar S, Perkins TJ, Rudnicki MA, Allan DS, Yoder MC, Suuronen EJ, Brand M. Epigenetic Activation of Pro-angiogenic Signaling Pathways in Human Endothelial Progenitors Increases Vasculogenesis. Stem Cell Reports 2017 Nov 14;9(5):1573-1587. doi: 10.1016/j.stemcr.2017.09.009.

Lacour F, Vezin E, Bentzinger CF, Sincennes MC, Giordani L, Ferry A, Mitchell R, Patel K, Rudnicki MA, Chaboissier MC, Chassot AA, Le Grand F. R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways. Cell Rep. 2017 Mar 7;18(10):2320-2330. doi: 10.1016/j.celrep.2017.02.036. PubMed PMID: 28273449.

Sincennes MC, Wang YX, Rudnicki MA. Primary Mouse Myoblast Purification using Magnetic Cell Separation. Methods Mol Biol. 2017;1556:41-50. doi: 10.1007/978-1-4939-6771-1_3. PubMed PMID: 28247344.

Dumont NA, Rudnicki MA. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration. Methods Mol Biol. 2017;1560:179-188. doi: 10.1007/978-1-4939-6788-9_12. PubMed PMID: 28155153.

2016

Lukjanenko L, Jung MJ, Hegde N, Perruisseau-Carrier C, Migliavacca E, Rozo M, Karaz S, Jacot G, Schmidt M, Li L, Metairon S, Raymond F, Lee U, Sizzano F, Wilson DH, Dumont NA, Palini A, Fässler R, Steiner P, Descombes P, Rudnicki MA, Fan CM, von Maltzahn J, Feige JN, Bentzinger CF. Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice. Nat Med. 2016 Aug;22(8):897-905. doi: 10.1038/nm.4126. Epub 2016 Jul 4. PubMed PMID: 27376579.

Pasut A, Chang NC, Rodriguez UG, Faulkes S, Yin H, Lacaria M, Ming H, Rudnicki MA. Notch Signaling Rescues Loss of Satellite Cells Lacking Pax7 and Promotes Brown Adipogenic Differentiation. Cell Rep. 2016 Jul 12;16(2):333 43. doi: 10.1016/j.celrep.2016.06.001. Epub 2016 Jun 23. PubMed PMID: 27346341

Jahani-Asl A, Yin H, Soleimani VD, Haque T, Luchman HA, Chang NC, Sincennes MC, Puram SV, Scott AM, Lorimer IA, Perkins TJ, Ligon KL, Weiss S, Rudnicki MA, Bonni A. Control of glioblastoma tumorigenesis by feed-forward cytokine signaling. Nat Neurosci. 2016 Jun;19(6):798-806. doi: 10.1038/nn.4295. Epub 2016 Apr 25. PubMed PMID: 27110918

Harper SC, Brack A, MacDonnell S, Franti M, Olwin BB, Bailey BA, Rudnicki MA, Houser SR. Is Growth Differentiation Factor 11 a Realistic Therapeutic for Aging-Dependent Muscle Defects? Circ Res. 2016 Apr 1;118(7):1143-50; discussion 1150. doi: 10.1161/CIRCRESAHA.116.307962. PubMed PMID: 27034276

2015

Chihara D, Romer AI, Bentzinger CF, Rudnicki MA, Krauss RS. PAX7 is required for patterning the esophageal musculature. Skelet Muscle. 2015 Dec 3;5:39. doi: 10.1186/s13395-015-0068-0. eCollection 2015 Dec 3. PubMed PMID: 26635949; PubMed Central PMCID: PMC4668666.

Dumont NA, Wang YX, von Maltzahn J, Pasut A, Bentzinger CF, Brun CE, Rudnicki MA. Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division. Nat Med. 2015 Dec;21(12):1455-63. doi: 10.1038/nm.3990. Epub 2015 Nov 16. PubMed PMID: 26569381; PubMed Central PMCID: PMC4839960.

Dick SA, Chang NC, Dumont NA, Bell RA, Putinski C, Kawabe Y, Litchfield DW, Rudnicki MA, Megeney LA. Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells. Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):E5246-52. doi: 10.1073/pnas.1512869112. Epub 2015 Sep 8. PubMed PMID: 26372956; PubMed Central PMCID: PMC4586827.

Jones AE, Price FD, Le Grand F, Soleimani VD, Dick SA, Megeney LA, Rudnicki MA. Wnt/ß-catenin controls follistatin signalling to regulate satellite cell myogenic potential. Skelet Muscle. 2015 Apr 28;5:14. doi: 10.1186/s13395-015-0038-6. eCollection 2015 Apr 28. PubMed PMID: 25949788; PubMed Central PMCID: PMC4421991.

Price FD, von Maltzahn J, Bentzinger CF, Dumont NA, Yin H, Chang NC, Wilson DH, Frenette J, Rudnicki MA. Corrigendum: Inhibition of JAK-STAT signaling stimulates adult satellite cell function. Nat Med. 2015 Apr;21(4):414. doi: 10.1038/nm0415-414a. PubMed PMID: 25849274.

2014

Smid JK, Faulkes S, Rudnicki MA. Periostin induces pancreatic regeneration. Endocrinology. 2015 Mar;156(3):824-36. doi: 10.1210/en.2014-1637. Epub 2014 Dec 8. PubMed PMID: 25485969.

Price FD, von Maltzahn J, Bentzinger CF, Dumont NA, Yin H, Chang NC, Wilson DH, Frenette J, Rudnicki MA. Inhibition of JAK-STAT signaling stimulates adult satellite cell function. Nat Med. 2014 Oct;20(10):1174-81. doi: 10.1038/nm.3655. Epub 2014 Sep 7. Erratum in: Nat Med. 2015 Apr;21(4):414. Nat Med. 2014 Oct;(10):1217. PubMed PMID: 25194569; PubMed Central PMCID: PMC4191983.

Jiang C, Wen Y, Kuroda K, Hannon K, Rudnicki MA, Kuang S. Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy. Dis Model Mech. 2014 Aug;7(8):997-1004. doi: 10.1242/dmm.015917. Epub 2014 Jun 6. PubMed PMID: 24906372; PubMed Central PMCID: PMC4107328.

Bentzinger CF, von Maltzahn J, Dumont NA, Stark DA, Wang YX, Nhan K, Frenette J, Cornelison DD, Rudnicki MA. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol. 2014 Apr 14;205(1):97-111. doi: 10.1083/jcb.201310035. Epub 2014 Apr 7. PubMed PMID: 24711502; PubMed Central PMCID: PMC3987134.

2013

von Maltzahn J, Zinoviev R, Chang NC, Bentzinger CF, Rudnicki MA. A truncated Wnt7a retains full biological activity in skeletal muscle. Nat Commun. 2013;4:2869. doi: 10.1038/ncomms3869. PubMed PMID: 24287629; PubMed Central PMCID: PMC3868162.

He WA, Berardi E, Cardillo VM, Acharyya S, Aulino P, Thomas-Ahner J, Wang J, Bloomston M, Muscarella P, Nau P, Shah N, Butchbach ME, Ladner K, Adamo S, Rudnicki MA, Keller C, Coletti D, Montanaro F, Guttridge DC. NF-?B-mediated Pax7 dysregulation in the muscle microenvironment promotes cancer cachexia. J Clin Invest. 2013 Nov;123(11):4821-35. PubMed PMID: 24084740; PubMed Central PMCID: PMC3809785.

von Maltzahn J, Jones AE, Parks RJ, Rudnicki MA. Pax7 is critical for the normal function of satellite cells in adult skeletal muscle. Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16474-9. doi: 10.1073/pnas.1307680110. Epub 2013 Sep 24. PubMed PMID: 24065826; PubMed Central PMCID: PMC3799311.

Liu W, Bi P, Shan T, Yang X, Yin H, Wang YX, Liu N, Rudnicki MA, Kuang S. miR-133a regulates adipocyte browning in vivo. PLoS Genet. 2013;9(7):e1003626. doi: 10.1371/journal.pgen.1003626. Epub 2013 Jul 11. PubMed PMID: 23874225; PubMed Central PMCID: PMC3708806.

Parker MH, von Maltzahn J, Bakkar N, Al-Joubori B, Ishibashi J, Guttridge D, Rudnicki MA. Retraction: MyoD-dependent regulation of NF-?B activity couples cell-cycle withdrawal to myogenic differentiation. Skelet Muscle. 2013 Jul 18;3(1):15. doi: 10.1186/2044-5040-3-15. PubMed PMID: 23870549; PubMed Central PMCID: PMC3717048.

Soleimani VD, Palidwor GA, Ramachandran P, Perkins TJ, Rudnicki MA. Chromatin tandem affinity purification sequencing. Nat Protoc. 2013 Aug;8(8):1525-34. doi: 10.1038/nprot.2013.088. Epub 2013 Jul 11. PubMed PMID: 23845964; PubMed Central PMCID: PMC4058862.

Wang YX, Bentzinger CF, Rudnicki MA. Treating muscular dystrophy by stimulating intrinsic repair. Regen Med. 2013 May;8(3):237-40. doi: 10.2217/rme.13.27. PubMed PMID: 23627818.

Pasut A, Jones AE, Rudnicki MA. Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. J Vis Exp. 2013 Mar 22;(73):e50074. doi: 10.3791/50074. PubMed PMID: 23542587; PubMed Central PMCID: PMC3639710.

Kuroda K, Kuang S, Taketo MM, Rudnicki MA. Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts. Skelet Muscle. 2013 Mar 5;3(1):5. doi: 10.1186/2044-5040-3-5. PubMed PMID: 23497616; PubMed Central PMCID: PMC3602004.

Campbell PA, Rudnicki MA. Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency. Stem Cells. 2013 Jun;31(6):1107-20. doi: 10.1002/stem.1365. PubMed PMID: 23495099.

Yin H, Pasut A, Soleimani VD, Bentzinger CF, Antoun G, Thorn S, Seale P, Fernando P, van Ijcken W, Grosveld F, Dekemp RA, Boushel R, Harper ME, Rudnicki MA. MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. Cell Metab. 2013 Feb 5;17(2):210-24. doi: 10.1016/j.cmet.2013.01.004. PubMed PMID: 23395168; PubMed Central PMCID: PMC3641657.

Price FD, Yin H, Jones A, van Ijcken W, Grosveld F, Rudnicki MA. Canonical Wnt signaling induces a primitive endoderm metastable state in mouse embryonic stem cells. Stem Cells. 2013 Apr;31(4):752-64. doi: 10.1002/stem.1321. PubMed PMID: 23307624.

Bentzinger CF, Wang YX, von Maltzahn J, Soleimani VD, Yin H, Rudnicki MA. Fibronectin regulates Wnt7a signaling and satellite cell expansion. Cell Stem Cell. 2013 Jan 3;12(1):75-87. doi: 10.1016/j.stem.2012.09.015. PubMed PMID: 23290138; PubMed Central PMCID: PMC3539137.

2012

Wang YX, Bentzinger CF, Rudnicki MA. Molecular regulation of determination in asymmetrically dividing muscle stem cells. Cell Cycle. 2013 Jan 1;12(1):3-4. doi: 10.4161/cc.23068. Epub 2012 Dec 19. PubMed PMID: 23255099; PubMed Central PMCID: PMC3570513.

von Maltzahn J, Renaud JM, Parise G, Rudnicki MA. Wnt7a treatment ameliorates muscular dystrophy. Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20614-9. doi: 10.1073/pnas.1215765109. Epub 2012 Nov 26. PubMed PMID: 23185011; PubMed Central PMCID: PMC3528612.

Huh MS, Price O'Dea T, Ouazia D, McKay BC, Parise G, Parks RJ, Rudnicki MA, Picketts DJ. Compromised genomic integrity impedes muscle growth after Atrx inactivation. J Clin Invest. 2012 Dec;122(12):4412-23. doi: 10.1172/JCI63765. Epub 2012 Nov 1. PubMed PMID: 23114596; PubMed Central PMCID: PMC3533543.

Kawabe Y, Wang YX, McKinnell IW, Bedford MT, Rudnicki MA. Carm1 regulates Pax7 transcriptional activity through MLL1/2 recruitment during asymmetric satellite stem cell divisions. Cell Stem Cell. 2012 Sep 7;11(3):333-45. doi: 10.1016/j.stem.2012.07.001. Epub 2012 Aug 2. PubMed PMID: 22863532; PubMed Central PMCID: PMC3438319.

Liu QC, Zha XH, Faralli H, Yin H, Louis-Jeune C, Perdiguero E, Pranckeviciene E, Muñoz-Cànoves P, Rudnicki MA, Brand M, Perez-Iratxeta C, Dilworth FJ. Comparative expression profiling identifies differential roles for Myogenin and p38a MAPK signaling in myogenesis. J Mol Cell Biol. 2012 Dec;4(6):386-97. doi: 10.1093/jmcb/mjs045. Epub 2012 Jul 30. PubMed PMID: 22847234; PubMed Central PMCID: PMC4580549.

Soleimani VD, Yin H, Jahani-Asl A, Ming H, Kockx CE, van Ijcken WF, Grosveld F, Rudnicki MA. Snail regulates MyoD binding-site occupancy to direct enhancer switching and differentiation-specific transcription in myogenesis. Mol Cell. 2012 Aug 10;47(3):457-68. doi: 10.1016/j.molcel.2012.05.046. Epub 2012 Jul 5. PubMed PMID: 22771117; PubMed Central PMCID: PMC4580277.

Rudnicki MA. Margaret Buckingham, discoveries in skeletal and cardiac muscle development, elected to the National Academy of Science. Skelet Muscle. 2012 Jun 7;2(1):12. doi: 10.1186/2044-5040-2-12. PubMed PMID: 22676886; PubMed Central PMCID: PMC3407767.

Soleimani VD, Punch VG, Kawabe Y, Jones AE, Palidwor GA, Porter CJ, Cross JW, Carvajal JJ, Kockx CE, van IJcken WF, Perkins TJ, Rigby PW, Grosveld F, Rudnicki MA. Transcriptional dominance of Pax7 in adult myogenesis is due to high-affinity recognition of homeodomain motifs. Dev Cell. 2012 Jun 12;22(6):1208-20. doi: 10.1016/j.devcel.2012.03.014. Epub 2012 May 17. PubMed PMID: 22609161; PubMed Central PMCID: PMC3376216.

Parker MH, von Maltzahn J, Bakkar N, Al-Joubori B, Ishibashi J, Guttridge D, Rudnicki MA. MyoD-dependent regulation of NF-?B activity couples cell-cycle withdrawal to myogenic differentiation. Skelet Muscle. 2012 May 19;2(1):6. doi: 10.1186/2044-5040-2-6. PubMed PMID: 22541644; PubMed Central PMCID: PMC3356597.

Yip DJ, Corcoran CP, Alvarez-Saavedra M, DeMaria A, Rennick S, Mears AJ, Rudnicki MA, Messier C, Picketts DJ. Snf2l regulates Foxg1-dependent progenitor cell expansion in the developing brain. Dev Cell. 2012 Apr 17;22(4):871-8. doi: 10.1016/j.devcel.2012.01.020. PubMed PMID: 22516202; PubMed Central PMCID: PMC4580287.

Pasut A, Rudnicki MA. The long, the short, and the micro: a polyA tale of Pax3 in satellite cells. Cell Stem Cell. 2012 Mar 2;10(3):237-8. doi: 10.1016/j.stem.2012.02.009. PubMed PMID: 22385651; PubMed Central PMCID: PMC3300070.